Biodentine stimulates the migratory and biological responses of human gingival fibroblast.
Abstract
Introduction: Biodentine (BD), a dentin substitute, is currently used to treat external cervical root resorption, but its effects on gingival fibroblasts (GFs) are not fully known.
Objective: To investigate and compare BD and MTA (mineral trioxide aggre-gate) in terms of proliferative, migratory, and adhesion effects on human pulpal and gingival cells.
Material and Methods: Cells were incubated directly on the surface of BD and MTA disks. Adhesion (4 and 24 h) and proliferation (3, 5, 7, 14, 21) were evaluated with crystal violet and MTT assays (n=9 X each group). A wound-healing assay was performed for cell migration, with 0.2 and 2 µg/ml MTA or BD (n=6 X each group). The cut-off point for statistical significance was set at p<0.05, p<0.01 and p<0.001.
Results: The best adhesion and proliferation results for gingival fibroblast (GFs) were obtained with BD (p<0.01). MTA and BD enhanced the migration of GFs in a dose-dependent manner, with superior results with BD, and 2 µg/ml was the optimal concentration for enhancing the migration of GFs.
Conclusion: Results indicate that BD and MTA exhibit excellent compatibility in terms of cell adhesion, proliferation, and cellular migration. Also, the results suggested that BD is associated with better results than MTA in GFs. The results support the clinical application of BD in areas colonized with GFs.
References
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[23]. A Saberi E, Farhadmollashahi N, Ghotbi F, Karkeabadi H, Havaei R. Cytotoxic effects of mineral trioxide aggregate, calcium enrichedmixture cement, Biodentine and octacalcium pohosphate onhuman gingival fibroblasts. J Dent Res Dent Clin Dent Prospects. 2016;10(2):75-80. doi: 10.15171/joddd.2016.012. PMID: 27429722; PMCID: PMC4946003.
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[31]. Jeong SY, Lee S, Choi WH, Jee JH, Kim HR, Yoo J. Fabrication of Dentin-Pulp-Like Organoids Using Dental-Pulp Stem Cells. Cells. 2020;9(3):642. doi: 10.3390/cells9030642. PMID: 32155898; PMCID: PMC7140482.
[32]. Chalisserry EP, Nam SY, Park SH, Anil S. Therapeutic potential of dental stem cells. J Tissue Eng. 2017; 8: 2041731417702531. doi: 10.1177/2041731417702531. PMID: 28616151; PMCID: PMC5461911.
[33]. Luo Z, Li D, Kohli MR, Yu Q, Kim S, He WX. Effect of Biodentine™ on the proliferation, migration and adhesion of human dental pulp stem cells. J Dent. 2014;42(4):490-7. doi: 10.1016/j.jdent.2013.12.011. PMID: 24440605.
[34]. Laurent P, Camps J, About I. Biodentine(TM) induces TGF-β1 release from human pulp cells and early dental pulp mineralization. Int Endod J. 2012;45(5):439-48. doi: 10.1111/j.1365-2591.2011.019 95.x. PMID: 22188368.
[2]. Jitaru S, Hodisan I, Timis L, Lucian A, Bud M. The use of bioceramics in endodontics - literature review. Clujul Med. 2016;89(4):470-473. doi: 10.15386/cjmed-612. PMID: 27857514; PMCID: PMC5111485.
[3]. Raghavendra SS, Jadhav GR, Gathani KM, Kotadia P. Bioceramics in endodontics - a review. J Istanb Univ Fac Dent. 2017;51(3 Suppl 1):S128-S137. doi: 10.17096/jiufd.63659. PMID: 29354316; PMCID: PMC5750835.
[4]. Kaur M, Singh H, Dhillon JS, Batra M, Saini M. MTA versus Biodentine: Review of Literature with a Comparative Analysis. J Clin Diagn Res. 2017;11(8):ZG01-ZG05. doi: 10.7860/JCDR/2017/25840.10374. PMID: 28969295; PMCID: PMC5620936.
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[6]. Zafar K, Jamal S, Ghafoor R. Bio-active cements-Mineral Trioxide Aggregate based calcium silicate materials: a narrative review. J Pak Med Assoc. 2020;70(3):497-504. doi: 10.5455/JPMA.16942. PMID: 32207434.
[7]. Pérard M, Le Clerc J, Watrin T, Meary F, Pérez F, Tricot-Doleux S, Pellen-Mussi P. Spheroid model study comparing the biocompatibility of Biodentine and MTA. J Mater Sci Mater Med. 2013;24(6):1527-34. doi: 10.1007/s10856-013-4908-3. Erratum in: J Mater Sci Mater Med. 2013;24(9):2275. Watrin, Tanguy [added]. PMID: 23515903.
[8]. Koubi G, Colon P, Franquin JC, Hartmann A, Richard G, Faure MO, Lambert G. Clinical evaluation of the performance and safety of a new dentine substitute, Biodentine, in the restoration of posterior teeth - a prospective study. Clin Oral Investig. 2013;17(1):243-9. doi: 10.1007/s00784-012-0701-9. PMID: 22411260; PMCID: PMC3536989.
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[12]. Mahmoud SH, El-Negoly SA, Zaen El-Din AM, El-Zekrid MH, Grawish LM, Grawish HM, Grawish ME. Biodentine versus mineral trioxide aggregate as a direct pulp capping material for human mature permanent teeth - A systematic review. J Conserv Dent. 2018;21(5):466-473. doi: 10.4103/JCD.JCD_198_18. PMID: 30294104; PMCID: PMC6161524.
[13]. Willershausen B, Marroquín BB, Schäfer D, Schulze R. Cytotoxicity of root canal filling materials to three different human cell lines. J Endod. 2000;26(12):703-7. doi: 10.1097/00004770-200012000-00007. PMID: 11471637.
[14]. Tai KW, Huang FM, Chang YC. Cytotoxic evaluation of root canal filling materials on primary human oral fibroblast cultures and a permanent hamster cell line. J Endod. 2001;27(9):571-3. doi: 10.1097/00004770-200109000-00004. PMID: 11556560.
[15]. De la Rosa-Ruiz MDP, Álvarez-Pérez MA, Cortés-Morales VA, Monroy-García A, Mayani H, Fragoso-González G, Caballero-Chacón S, Diaz D, Candanedo-González F, Montesinos JJ. Mesenchymal Stem/Stromal Cells Derived from Dental Tissues: A Comparative In Vitro Evaluation of Their Immunoregulatory Properties Against T cells. Cells. 2019;8(12):1491. doi: 10.3390/cells8121491. PMID: 31766697; PMCID: PMC6953107.
[16]. Zhou HM, Shen Y, Wang ZJ, Li L, Zheng YF, Häkkinen L, Haapasalo M. In vitro cytotoxicity evaluation of a novel root repair material. J Endod. 2013;39(4):478-83. doi: 10.1016/j.joen.2012.11.026. PMID: 23522540.
[17]. Brenes-Valverde K, Conejo-Rodríguez E, Vega-Baudrit JR, Montero-Aguilar M, Chavarría-Bolaños D. Evaluation of Microleakage by Gas Permeability and Marginal Adaptation of MTA and Biodentine™ Apical Plugs: In Vitro Study. Odovtos-International J Dent Scie. 2018;20(1):57-67.
[18]. Nowicka A, Wilk G, Lipski M, Kołecki J, Buczkowska-Radlińska J. Tomographic Evaluation of Reparative Dentin Formation after Direct Pulp Capping with Ca(OH)2, MTA, Biodentine, and Dentin Bonding System in Human Teeth. J Endod. 2015;41(8):1234-40. doi: 10.1016/j.joen.2015.03.017. PMID: 26031301.
[19]. Woloszyk A, Buschmann J, Waschkies C, Stadlinger B, Mitsiadis TA. Human Dental Pulp Stem Cells and Gingival Fibroblasts Seeded into Silk Fibroin Scaffolds Have the Same Ability in Attracting Vessels. Front Physiol. 2016;7:140. doi: 10.3389/fphys.2016.00140. PMID: 27148078; PMCID: PMC4835714.
[20]. Hasweh N, Awidi A, Rajab L, Hiyasat A, Jafar H, Islam N, Hasan M, Abuarqoub D. Characterization of the biological effect of BiodentineTM on primary dental pulp stem cells. Indian J Dent Res. 2018;29(6):787-793. doi: 10.4103/ijdr.IJDR_28_18. PMID: 30589009.
[21]. Rajasekharan S, Martens LC, Cauwels RG, Verbeeck RM. Biodentine™ material characteristics and clinical applications: a review of the literature. Eur Arch Paediatr Dent. 2014;15(3):147-58. doi: 10.1007/s40368-014-0114-3. PMID: 24615290.
[22]. Corral Nuñez CM, Bosomworth HJ, Field C, Whitworth JM, Valentine RA. Biodentine and mineral trioxide aggregate induce similar cellular responses in a fibroblast cell line. J Endod. 2014;40(3):406-11. doi: 10.1016/j.joen.2013.11.006. PMID: 24565661.
[23]. A Saberi E, Farhadmollashahi N, Ghotbi F, Karkeabadi H, Havaei R. Cytotoxic effects of mineral trioxide aggregate, calcium enrichedmixture cement, Biodentine and octacalcium pohosphate onhuman gingival fibroblasts. J Dent Res Dent Clin Dent Prospects. 2016;10(2):75-80. doi: 10.15171/joddd.2016.012. PMID: 27429722; PMCID: PMC4946003.
[24]. Mozayeni MA, Milani AS, Marvasti LA, Asgary S. Cytotoxicity of calcium enriched mixture cement compared with mineral trioxide aggregate and intermediate restorative material. Aust Endod J. 2012; 38(2):70-5. doi: 10.1111/j.1747-4477.2010.00269.x. PMID: 22827819.
[25]. Fridland M, Rosado R. MTA solubility: a long term study. J Endod. 2005;31(5):376-9. doi: 10.1097/01.don.0000140566.97319.3e. PMID: 15851933.
[26]. Siboni F, Taddei P, Zamparini F, Prati C, Gandolfi MG. Properties of BioRoot RCS, a tricalcium silicate endodontic sealer modified with povidone and polycarboxylate. Int Endod J. 2017;50 Suppl 2:e120-e136. doi: 10.1111/iej.12856. PMID: 28881478.
[27]. Flores DS, Rached FJ Jr, Versiani MA, Guedes DF, Sousa-Neto MD, Pécora JD. Evaluation of phy-sicochemical properties of four root canal sealers. Int Endod J. 2011;44(2):126-35. doi: 10.1111/j.1365-2591.2010.01815.x. PMID: 21091494
[28]. Kramer N, Walzl A, Unger C, Rosner M, Krupitza G, Hengstschläger M, Dolznig H. In vitro cell migration and invasion assays. Mutat Res. 2013;752(1):10-24. doi: 10.1016/j.mrrev.2012.08.001. PMID: 22940039.
[29]. Zanini M, Sautier JM, Berdal A, Simon S. Biodentine induces immortalized murine pulp cell differentiation into odontoblast-like cells and stimulates biomineralization. J Endod. 2012;38(9):1220-6. doi: 10.1016/j.joen.2012.04.018. PMID: 22892739.
[30]. Shakoori P, Zhang Q, Le AD. Applications of Mesenchymal Stem Cells in Oral and Craniofacial Regeneration. Oral Maxillofac Surg Clin North Am. 2017;29(1):19-25. doi: 10.1016/j.coms.2016.08.009. PMID: 27890225.
[31]. Jeong SY, Lee S, Choi WH, Jee JH, Kim HR, Yoo J. Fabrication of Dentin-Pulp-Like Organoids Using Dental-Pulp Stem Cells. Cells. 2020;9(3):642. doi: 10.3390/cells9030642. PMID: 32155898; PMCID: PMC7140482.
[32]. Chalisserry EP, Nam SY, Park SH, Anil S. Therapeutic potential of dental stem cells. J Tissue Eng. 2017; 8: 2041731417702531. doi: 10.1177/2041731417702531. PMID: 28616151; PMCID: PMC5461911.
[33]. Luo Z, Li D, Kohli MR, Yu Q, Kim S, He WX. Effect of Biodentine™ on the proliferation, migration and adhesion of human dental pulp stem cells. J Dent. 2014;42(4):490-7. doi: 10.1016/j.jdent.2013.12.011. PMID: 24440605.
[34]. Laurent P, Camps J, About I. Biodentine(TM) induces TGF-β1 release from human pulp cells and early dental pulp mineralization. Int Endod J. 2012;45(5):439-48. doi: 10.1111/j.1365-2591.2011.019 95.x. PMID: 22188368.
Published
2022-04-30
How to Cite
VÁZQUEZ-VÁZQUEZ, Febe et al.
Biodentine stimulates the migratory and biological responses of human gingival fibroblast..
Journal of Oral Research, [S.l.], v. 11, n. 2, p. 1-14, apr. 2022.
ISSN 0719-2479.
Available at: <https://www.joralres.com/index.php/JOralRes/article/view/joralres.2022.019>. Date accessed: 26 apr. 2024.
doi: https://doi.org/10.17126/joralres.2022.019.
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