Relationship between percentage of regulatory T-cells and dental amalgam fillings.

  • Fahad Khaliq Department of Oral and Maxillofacial Surgery, Nishtar Institute of Dentistry Multan.
  • Nadeem Afzal Department of Immunology, University of Health Sciences Lahore.
  • Muhammad Kashif Department of Immunology, University of Health Sciences Lahore.
  • Faheem Shahzad Department of Immunology, University of Health Sciences Lahore.
DOI: https://doi.org/10.17126/joralres.2016.034

Abstract

Introduction: Regulatory T-cells are the main component of peripheral tolerance and their level is decreased in autoimmunity. In dental amalgam, a mixture of metals is used as a restorative material. During daily activities, these metals are ingested and affect renal, neurosensory and immune systems. Studies have demonstrated an increased risk of autoimmune diseases in patients with dental amalgam fillings. It was hypothesized that the percentage of regulatory T-cells decreases in individuals with amalgam fillings. Therefore this study was designed to determine and compare the percentage of regulatory T-cells in individuals with and without amalgam fillings. Material and Methods: This was a cross-sectional study. Subjects were divided into two groups with each group consisting of 40 individuals. Group I (study group) comprised individuals with amalgam fillings, and Group II (control group), individuals without amalgam fillings in their teeth. Blood samples of all the participants were collected and tagged with CD4‑FITC, CD25‑PE and CD127‑PerCP-Cy monoclonal antibodies for the detection of regulatory T-cells, FACSCalibur was used for this purpose. Results: The percentage of regulatory T-cells in the control group was high (77.77 ± 5.54 %) compared to the study group (76.09 ± 7.68 %), however, on comparison, the difference was not statistically significant (p=0.25). Conclusion: Dental amalgam fillings did not show a declining effect on the percentage of regulatory T-cells.

 

References

1. Ndhlovu LC, Leal FE, Eccles-James IG, Jha AR, Lanteri M, Norris PJ, Barbour JD, Wachter DJ, Andersson J, Taskén K, Torheim EA, Aandahl EM, Kallas EG, Nixon DF. A novel human CD41 T-cell inducer subset with potent immunostimulatory properties. Eur J Immunol. 2010; 40:134–41.
2. Edinger M. Driving tolerance: CAR expressing Tregs for tolerance induction in organ and stem cells transplantation. J Clin Investig. 2016; 126(4):1248-50.
3. Jergovic M, Bendelja K,Vidovic A, Vojvoda V, Aberle N, Rabatic S, Jovanovic T, Sabioncello A. Patients with posttraumatic stressdisorder exhibit an altered phenotype of regulatory T cells. Allergy Asthma Clin Immunol. 2014; 10:43.
4. Bharti R, Wadhwani KK, Tikku AP, Chandra A. Dental amalgam: An update. J Conserv Dent. 2010;13(4):204-8.
5. Rathore M, Singh A, Pant VA. The Dental Amalgam Toxicity Fear: A Myth or Actuality. Toxicol Inter. 2012;19(2):81-8.
6. Yalcin-Cakir F, Ergin E, Gurgan S, Sabuncuoglu S, Arpa CS, Tokgoz İ, Ozgunes H, Kiremitci A. Effect of Bleaching on Mercury Release from Amalgam Fillings and Antioxidant Enzyme Activities: A Pilot Study. J Esthet Restor Dent. 2015; 27: 29–36.
7. Mortazavi M, Mortazavi SMJ. Increased mercury release from dental amalgam restorations after exposure to electromagnetic field as potential hazard to hypersensitive people and pregnant females. The three modern faces of mercury. Rev Environ Health. 2015; 30(4):287–92.
8. John K, Just A, Aschner M. What Is the Risk? Dental Amalgam, Mercury Exposure, and Human Health Risks Throughout the Life Span. Epigenetics, the Environment, and Children’s Health Across Lifespans. Switzerland: Springer International Publishing; 2016.
9. Linders J, Janssen C, Testai E, Vighi M, Dekant W, Munth J, Pirrone N, Richardson M. Opinion on environmental risks and indirect health effects of mercury from dental amalgam. Reg Toxicol Pharmacol 2015; 72: 85-6.
10. Woods JS, Heyer NJ, Russo JE, Martin MD, Pillai PB, Farin FM. Modification of neurobehavioral effects of mercury by genetic polymorphisms of metallothionein in children. Neurotoxicol Teratol. 2013; 39: 36-44.
11. Stejskal, V. The role of heavy metals in autoimmune diseases. Adv Integ Med. 2014; 1(3): 156-7.
12. Cojocaru M, Chicos B. The role of heavy metals in autoimmunity. Rom J Inter Med. 2014; 52(3): 189-91.
13. Huss DJ, Pellerin AF, Collette BP, Kannan AK, Peng L, Datta A, Wipke BT1, Fontenot JD. Anti‐CD25 monoclonal antibody Fc variants differentially impact Treg cells and immune homeostasis. Immunology. 2016 [Epub ahead of print]
14. Boyce C, Lane Y, Hingorani R, McIntyre C, Ruitenberg J, Ghanekar S. 2010. Human Regulatory T-Cell Isolation and Measurement of Function. BD Biosciences, Application Notes. [accessed 2016 February 02] Available at: https://www.bdbiosciences.com/documents/Human_Reg_Tcell_Isolation_&_Measurement.pdf.
15. Ammirati E, Cianflone D, Banfi M. Circulating CD4+CD25hiCD127lo Regulatory T cells levels do not reflect the severity or extent of carotid and coronary atherosclerosis. Arterioscler Thromb Vasc Biology. 2010; 30:1832-41.
16. Hoffmann HJ, Malling TM, Topcu A, Ryder LP, Nielsen KR, Varming K, Dahl R, Omland O, Sigsgaard T. CD4dimCD25bright Treg Cell Frequencies above a Standardized Gating Threshold are Similar in Asthmatics and Controls. Cytometry A. 2007; 71(6):371–8.
17. Liu W, Putnam AL, Xu-yu Z, Szot GL, Lee MR, Zhu S, Gottlieb PA, Kapranov P, Gingeras TR, Fazekas de St Groth B, Clayberger C, Soper DM, Ziegler SF, Bluestone JA. CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ Treg cells. J Exp Med. 2006; 203(7):1701-11.
18. Thongpan M, Kitiyakara C, Louischaroen Y, Keesukphan P, Chaiyaroj SC. Analysis of Foxp3, CD25, and CD127 Expressed on Regulatory T Cells in Thai Subjects. Asian Pacific J Allergy Immunol. 2009; 27:137-45.
19. Loftenius A, Englund SG, Ekstrand J. Acute exposure to mercury from amalgam: no short-time effect on the peripheral blood lymphocytes in healthy individuals. J Toxicol Environ Health. 1998; 7:547-60.
20. Osorio E, Toledano M, Bravo M, Osorio R. Short-term changes in lymphocytes after placement of silver amalgam restorations in healthy subjects. Dent Mater. 1995; 11:323-6.
21. Wilhelm M, Dünninger P, Rüppel R, Tony HP, Wilms K, Klaiber B. Failure to detect any effect of amalgam restorations on peripheral blood lymphocyte populations. Clini Investig. 1992; 70(9):728-34.
22. Shenker BJ, Maserejian NN, Zhang A, McKinlay S. Immune Function Effects of Dental Amalgam in Children: A Randomized Clinical Trial. JAMA. 2008; 139(11):1496–505.
23. DiPietro A, Visalli G, La Maestra S, Micale R, Baluce B, Matarese G, Cingano L, Scoglio ME. Biomonitoring of DNA damage in peripheral blood lymphocytes of subjects with dental restorative fillings. Mutat Res. 2008; 650:115–22.
24. Park SH, Araki S, Nakata A, Kim YH, Park JA, Tanigawa T, Yokoyama K, Sato H. Effects of occupational metallic mercury vapour exposure on suppressor-inducer (CD4+CD45RA+) T lymphocytes and CD57+CD16+ natural killer cells. Inter Arch Occup Environ Health. 2000; 73(8):537-42.
25. Eggleston DW. Effect of dental amalgam and nickel alloys on T-lymphocytes. J Prosthet Dent. 1984; 51(5):617-23.
Published
2016-06-13
How to Cite
KHALIQ, Fahad et al. Relationship between percentage of regulatory T-cells and dental amalgam fillings.. Journal of Oral Research, [S.l.], v. 5, n. 4, p. 153-158, june 2016. ISSN 0719-2479. Available at: <https://www.joralres.com/index.php/JOralRes/article/view/joralres.2016.034>. Date accessed: 07 may 2024. doi: https://doi.org/10.17126/joralres.2016.034.
Citation Formats
Issue
Vol 5 No 4
Section
Articles

Keywords

Regulatory T-cells; peripheral tolerance; autoimmunity; dental amalgam.

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