Regenerating effect of hyaluronic acid on circumferential bone defects in Wistar albino rats.


Objective: To determine the regenerating effect of hyaluronic acid on circumferential bone defects in albino Wistar rats. Material and Methods: An experimental type study was designed and carried out with 15 albino male Wistar rats, 4 months old and weighing between 250 and 350 grams. Two circumferential bone defects 3mm in diameter and 0.8mm deep were created in the calvaria of the parietal bone (on both sides of the midline). One defect was filled with a demineralized bone matrix (control group); while the other defect was filled with the combination of a demineralized bone matrix plus hyaluronic acid (experimental group). Five experimental rats were euthanized at 30, 60 and 90 days after surgery and they were histologically evaluated following the parameters proposed by Heiple. Results: The experimental group presented a better degree of bone regeneration at 30 and 60 postoperative days. Conclusion: Hyaluronic acid is effective in bone regeneration of circumferential bone defects.


1. Príncipe-Delgado Y, Mallma-Medina A, Castro-Rodríguez Y. Efectividad del plasma rico en fibrina y membrana de colágeno en la regeneración ósea guiada. Rev. Clin. Periodoncia Implantol. Rehabil. Oral. 2019; 12(2): 63-65.
2. Gjermo P, Rösing CK, Susin C, Oppermann R. Periodontal diseases in Central and South America. Periodontol 2000. 2002; 29:70-78.
3. Pardo F, Hernández L. Enfermedad periodontal: enfoques epidemiológicos para su análisis como problema de salud pública. Rev. Salud Pública. 2018; 20 (2): 258-64.
4. Carvajal P. Enfermedades periodontales como un problema de salud pública: el desafío del nivel primario de atención en salud. Rev Clin. Periodoncia Implantol Rehabil Oral. 2016; 9(2): 177-83
5. Martinez O, Barone A, Covani U, Fernández A, Jiménez A, Monsalve L, Velasco E. Injertos óseos y biomateriales en implantologia oral. Avances en Odontoestomatología. 2018; 34(3): 111-9.
6. Dias F, Arias A, Borie E, Lezcano M, Arellano-Villalón M, Fuentes R. Comparison of Morphological Quantitative Characteristics of the Newly Formed Alveolar Bone After the Application of Demineralized Bone Matrix and Cortical Bone Allografts. Int. J. Morphol. 2019; 37(4): 1509-16.
7. Tolg C, McCarthy JB, Yazdani A, Turley EA. Hialuronano y RHAMM en la reparación de heridas y la 'cancerización' de los tejidos del estroma. Biomed Res Int 2014; 2014: 1-18.
8. Kutlubay Z, Engin B, Serdaroǧlu S, Tüzün Y. Hyalüronikasitdolgular. Dermatoz. 2011; 2: 255-60
9. National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. 8th edition. Washington (DC): National Academies Press (US); 2011.
10. BioProPeru: Productos Osteobiológicos. Avalaible at:
11. Heiple KG, Chase SW, Herndon CH. A comparative study of the healing process following different types of bone transplantation. J Bone Joint Surg Am 1963; 45:1593-1616.
12. Mani A, Pawar B, Pendyala G, Mustilwar R, Bhosale A, Bhadange S. Hyaluronic acid - A boon to periodontal therapy. Pravara Med Rev. 2016; 8(1):8-13.
13. Diker N, Gulsever S, Koroglu T, Yilmaz Akcay E, Oguz Y. Effects of Hyaluronic Acid and Hydroxyapatite/Beta-tricalcium Phosphate in Combination on Bone Regeneration of a Critical-size Defect in an Experimental Model. J Craniofac Surg. 2018; 29(4):1087-1093
14. Agrali OB, Yildirim S, Ozener HO, Köse KN, Ozbeyli D, Soluk-Tekkesin M, Kuru L. Evaluation of the Effectiveness of Esterified Hyaluronic Acid Fibers on Bone Regeneration in Rat Calvarial Defects. Biomed Res Int. 2018: 3874131.
15. Koca C, Komerik N, Ozmen O. Comparison of efficiency of hyaluronic acid and/or bone grafts in healing of bone defects. Niger J Clin Pract. 2019; 22(6):754-762.
16. Taz M, Makkar P, Imran KM, Jang DW, Kim YS, Lee BT. Bone regeneration of multichannel biphasic calcium phosphate granules supplemented with hyaluronic acid. Mater Sci Eng C Mater Biol Appl. 2019;99:1058-66
17. Cai Z, Feng Y, Li C, Yang K, Sun T, Xu L, Chen Y, Yan CH, Lu WW, Chiu KY. Magnoflorine with hyaluronic acid gel promotes subchondral bone regeneration and attenuates cartilage degeneration in early osteoarthritis. Bone. 2018;116:266-78.
18. Matheus HR, Ervolino E, Gusman DJR, Alves BES, Fiorin LG, Pereira PA, de Almeida JM. Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects. J Periodontol. 2020.
How to Cite
BUENO-BELTRÁN, Cecilia et al. Regenerating effect of hyaluronic acid on circumferential bone defects in Wistar albino rats.. Journal of Oral Research, [S.l.], v. 10, n. 4, p. 1-11, aug. 2021. ISSN 0719-2479. Available at: <>. Date accessed: 18 jan. 2022. doi: