Gene expression of collagen type I alpha 2 and its relationship with dental fluorosis.

  • Bertha Gastelbondo-Pastrana Prometeus & Biomedicine Research Group applied to Clinical Sciences, Universidad de Cartagena, Colombia.
  • Meisser Vidal Madera Anaya Prometeus & Biomedicine Research Group applied to Clinical Sciences, Universidad de Cartagena, Colombia. Research Department, School of Dentistry, Universidad de Cartagena, Colombia.
  • Amileth Suárez-Causado Prometeus & Biomedicine Research Group applied to Clinical Sciences, Universidad de Cartagena, Colombia. Department of Basic Sciences, Biochemistry Unit, School of Medicine, Universidad de Cartagena, Colombia.

Abstract

Objective: To compare the gene expression levels of collagen type I alpha 2 (COL1A2) in children with and without dental fluorosis. Methods: Cross-sectional study involving 92 children between 5 and 12 years of age. Socio-demographic characteristics, the presence of dental fluorosis by means of the Thylstrup-Fejerskov index, and gene expression analysis of COL1A2 in peripheral blood samples by reverse transcription polymerase chain reaction (RT-PCR) assays, were described. For the descriptive analysis, measures of central tendency, dispersion and proportions were used. Differences between the groups (p<0.05) were established by the Student t-test. Results: Mean age was 8.6 (SD=1.9) years, 51.1% were female; 54 children were diagnosed with fluorosis and 38 without fluorosis; prevalence of dental fluorosis was 58.7% (95% CI: 48.4% -68.9%). Gene expression of COL1A2 was statistically significantly lower (p<0.05) in the participants with dental fluorosis. Conclusion: There are differences in the expression levels of the COL1A2 gene among the population under study. Therefore, COL1A2 may be potentially involved in the development of dental fluorosis.

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Published
2018-07-30
How to Cite
GASTELBONDO-PASTRANA, Bertha; MADERA ANAYA, Meisser Vidal; SUÁREZ-CAUSADO, Amileth. Gene expression of collagen type I alpha 2 and its relationship with dental fluorosis.. Journal of Oral Research, [S.l.], v. 7, n. 6, p. 232-235, july 2018. ISSN 0719-2479. Available at: <http://www.joralres.com/index.php/JOR/article/view/joralres.2018.056>. Date accessed: 28 oct. 2020. doi: https://doi.org/10.17126/joralres.2018.056.
Section
Communications

Keywords

dental fluorosis; dental enamel; gene expression; collagen.