Salivary stimulation by prolonged release films of pilocarpine in diabetic rats.

Jesús Israel Rodríguez, Rosa Isela Sánchez, Marianela Garza, María Argelia Nakagoshi, Juan Manuel Solis, Katiushka Arévalo, Emmanuel Garza


Introduction: The local use of prolonged drug delivery in the oral cavity provides many advantages, increasing the pharmacologic action in the local desirable site, the reduction of the usual dose and reduced adverse effects. Pilocarpine is a cholinergic drug approved by the FDA for the treatment of glandular hypofunction, however the diversity of the adverse effects limit their use.Objective: To evaluate the cytotoxicity of biofilms in adherent fibroblasts and their ability to release pilocarpine in vivo for prolonged time in the oral cavity of diabetic rats and the effect on salivary flow.Methods: Chitosan and HPMC (Methocel K4MCR) biofilms were prepared in 1% acetic acid, added with pilocarpine by magnetic stirring. The cytotoxicity of  biofilms was evaluated in adherent fibroblasts HS27 and assessed by neutral red technique. The sialogogue effect of biofilms was evaluated on the floor of the mouth in diabetic rats, then performing a histopathological analysis using hematoxylin and eosin staining and Masson trichrome. Results: Biofilms were biocompatible with 96% cell viability. It was posible to increase the stimulation of salivary flow in diabetic rats (6.36±0.987 mg/hr) compared to the control group (0.5±0.06 mg/hr). Histopathological analysis showed no inflammatory infiltrate present in the area of application of biofilms. Conclusion: Biofilms were biocompatible with high cell viability, also managed to considerably increase salivary flow in diabetic rats, without triggering an inflammatory infiltrate in the area of application, indicating that it is a product of sustained release biocompatible and safe for pilocarpine administration.


Hyposalivation, Cytotoxicity, Rats, Pilocarpine, Biofilms.


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