Evaluation in vitro of cytotoxicity of dentinal desensitizing on human gingival fibroblasts.

Diego Antonio Vergara, Claudio Andres Villegas, María Francisca Pavicic, Marco Antonio Fritz, Ingrid Pamela Ehrenfeld


Introduction: The purpose of this study is to compare the cytotoxic effect of three materials which have been used for the treatment of dental hypersensitivity. Material and method: In-vitro study. Dentinal desensitizing Clinpro (3M ESPE), Seal&Protect (Dentsply) and UltraEZ (Ultradent) were used at concentrations of 0.1; 0.05; 0.01 and 0.001 g/ml against human gingival fibroblasts. Furthermore, Clinpro and Seal&Protect were applied to this cell culture as polymerized discs. Toxicity was assessed at 24 and 48 hours by cell viability assay (MTT). Statistical analysis for cell viability was performed using ANOVA two-ways and Tukey's post hoc test. Statistical significance was set at 5%. Results: Seal&Protect and Clinpro happened to be highly toxic at 24 and 48 hours, reaching 70% toxicity at concentrations over 0,01 g/ml. Seal&Protect and Clinpro polymerized discs were toxic after 24 and 48 hours. UltraEZ was increase of the cell viability between a 46% and 67% at 24 hours and between an 8% and 45% at 48 hours. Statistical analysis showed differences between these three desensitizing comparing concentration and control group (p<0,05). Discussion: UltraEZ does not have cytotoxic effect and may be considered a compatible and safe material to be utilized, whereas polymerized and non-polymerized Clinpro and Seal&Protect should be used with caution.

Keywords: “Dentin Desensitizing Agents”, “Cytotoxic”, “Human Gingival Fibroblasts”,    "Dentin Sensitivity".

Evaluación in vitro de la citotoxicidad de desensibilizantes dentinarios en fibroblastos gingivales humanos.

Introducción: El propósito de este estudio es comparar el efecto citotóxico de tres materiales que se han utilizado para el tratamiento de la hipersensibilidad dental. Material y método: Estudio in- vitro. Los desensibilizantes dentinarios Clinpro (3M ESPE), Seal&Protect (Dentsply) y UltraEZ (Ultradent) fueron utilizados a concentraciones de  0,1; 0,05; 0,01 y 0,001 g/ml sobre cultivos celulares de fibroblastos gingivales humanos. Además, Clinpro y Seal&Protect se aplicaron a este cultivo celular como discos polimerizados. La toxicidad se evaluó a 24 y 48 horas mediante ensayo de viabilidad (MTT). El análisis estadístico para la viabilidad celular se realizó mediante ANOVA de dos vías seguido de análisis Tukey. La significancia estadística se fijó al 5%. Resultados: Clinpro y Seal&Protect resultaron ser altamente tóxicos a las 24 y 48 horas, alcanzando un 70% de toxicidad a concentraciones superiores de 0,01 g/ml. Los discos polimerizados de Clinpro y Seal&Protect fueron tóxicos a 24 y 48 horas. UltraEZ produjo un aumento de la viabilidad celular entre un 46% y 67% a las 24 horas y entre un 8% y 45% a las  48 horas. El análisis estadístico mostró diferencias entre estos tres desensibilizantes al comparar la concentración y su grupo control (p<0,05). Discusión: UltraEZ no tuvo efecto citotóxico y puede ser considerado como un material compatible y seguro para ser utilizado, mientras que Clinpro y Seal&Protect en su estado polimerizado y  no polimerizado deberían ser utilizados con precaución.

Palabras clave: “Desensibilizante Dentinario”, “Citotoxicidad”, “Fibroblastos Gingivales Humanos”,  "Sensibilidad dentinaria".


West N, Seong J, Davies M. Dentine hypersensitivity. Monogr Oral Sci. 2014;(25):108-22.

Addy M, Urquhart E. Dentine hypersensitivity: its prevalence, aetiology and clinical management. Dent Update. 1992;19(10):407-8,410-412.

Rees JS, Addy M. A cross-sectional study of dentine hypersensitivity. J Clin Periodontol. 2002;29(11):997-1003.

Brannström M. The hydrodynamics of the dental tubule and pulp fluid: its significance in relation to dentinal sensitivity. Annu Meet Am Inst Oral Biol. 1966; 23:219.

Lin PY, Cheng YW, Chu CY, Chien KL, Lin CP, Tu YK. In-office treatment for dentin hypersensitivity: a systematic review and network meta-analysis. J Clin Periodontol. 2013;40(1):53-64.

Trushkowsky RD, Oquendo A. Treatment of dentin hypersensitivity. Dent Clin North Am. 2011;55(3):599-608.

Pradeep AR, Agarwal E, Naik SB, Bajaj P, Kalra N. Comparison of efficacy of three commercially available dentifrices [corrected] on dentinal hypersensitivity: a randomized clinical trial. Aus Dent J. 2012;57(4):429-434.

Sethna GD, Prabhuji ML, Karthikeyan BV. Comparison of two different forms of varnishes in the treatment of dentine hypersensitivity: a subject-blind randomised clinical study. Oral Health Prev Dent. 2011;9(2):143-150.

Da Rosa WL, Lund RG, Piva E, Da Silva AF. The effectiveness of current dentin desensitizing agents used to treat dental hypersensitivity: a systematic review. Quintessence Int. 2013;44(7) :535-546.

Ding YJ, Yao H, Wang GH, Song H. A randomized double-blind placebo-controlled study of the efficacy of Clinpro XT varnish and Gluma dentin desensitizer on dentin hypersensitivity. Am J Dent. 2014;27(2):79-83.

Sigusch BW, Pflaum T, Völpel A, Gretsch K, Hoy S, Watts DC, Jandt KD. Resin-composite cytotoxicity varies with shade and irradiance. Dent Mater. 2012;28(3):312-319.

Durner J, Wellner P, Hickel R, Reichl FX. Synergistic interaction caused to human gingival fibroblasts from dental monomers. Dent Mater. 2012;28(8):818-823.

Lee JH, Jung JY, Jeong YJ, Park JH, Yang KH, Choi NK, Kim SH, Kim WJ. Involvement of both mitochondrial- and death receptor-dependent apoptotic pathways regulated by Bcl-2 family in sodium fluoride-induced apoptosis of the human gingival fibroblasts. Toxicology. 2008;243(3):340-347.

Hoang-Dao BT, Hoang-Tu H, Tran-Hung L, Camps J, Koubi G, About I. Evaluation of a natural resin-based new material (Shellac F) as a potential desensitizing agent. Dent Mater. 2008;27(7):1001-1007.

Camps J, About I, Van Meerbeek B, Franquin JC. Efficiency and cytotoxicity of resin-based desensitizing agents. Am J Dent. 2002;15(5):300-304.

Sengun A, Buyukbas S, Hakki SS. Cytotoxic effects of dental desensitizers on human gingival fibroblasts. J Biomed Mater Res B Appl Biomater. 2006;78(1):131-137.

Furche S, Hickel R, Reichl FX, van Landuyt K, Shehata M, Durner J. Quantification of elutable substances from methacrylate based sealers and their cytotoxicity effect on with human gingival fibroblasts. Dent Mater. 2013;29(6):618-625.

Morrison B, Sidow S, McNally K, McPherson J, Chuang A. An in vitro evaluation of the growth of human periodontal ligament fibroblasts after exposure to a 4-META-containing methacrylate-based endodontic sealer. J Endod. 2011;37(6):803-806.

Guo LW, Wu Q, Green B, Nolen G, Shi L, Losurdo J, Deng H, Bauer S, Fang JL, Ning B. Cytotoxicity and inhibitory effects of low-concentration triclosan on adipogenic differentiation of human mesenchymal stem cells. Toxicol Appl Pharmacol. 2012;262(2):117-123.

Kim K, Son KM, Kwon JH, Lim BS, Yang HC. The effects of restorative composite resins on the cytotoxicity of dentine bonding agents. Dent Mater J. 2013;32(5):709-717.

Durner J, Wellner P, Hickel R, Reichl FX. Synergistic interaction caused to human gingival fibroblasts from dental monomers. Dent Mater. 2012;28(8):818-823.

Lad PP, Kamath M, Tarale K, Kusugal PB. Practical clinical considerations of luting cements: A review. J Int Oral Health. 2014;6(1):116-120.

Selimović-Dragaš M, Huseinbegović A, Kobašlija S, Hatibović-Kofman S. A comparison of the in vitro cytotoxicity of conventional and resin modified glass ionomer cements. Bosn J Basic Med Sci. 2012;12(4):273-278.

Khoroushi M, Keshani F. A review of glass-ionomers: From conventional glass-ionomer to bioactive glass-ionomer. Dent Res J. (Isfahan). 2013;10(4):411-420.

Cataldi A, Zara S, Rapino M, Patruno A, di Giacomo V. Human gingival fibroblasts stress response to HEMA: A role for protein kinase C α. J Biomed Mater Res A. 2013;101(2):378-384.

Szczepanska J, Poplawski T, Synowiec E, Pawlowska E, Chojnacki J, Blasiak J. 2-hydroxylethyl methacrylate (HEMA), a tooth restoration component, exerts its genotoxic effects in human gingival fibroblasts trough methacrylic acid, an immediate product of its degradation. Mol Biol Rep. 2012;39(2):1561-1574.

Di Nisio C, Zara S, Cataldi A, di Giacomo V. 2-Hydroxyethyl methacrylate inflammatory effects in human gingival fibroblasts. Int Endod J. 2013;46(5):466-476.

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