Evaluation in vitro of cytotoxicity of dentinal desensitizing on human gingival fibroblasts.

Diego Antonio Vergara, Claudio Andres Villegas, María Francisca Pavicic, Marco Antonio Fritz, Ingrid Pamela Ehrenfeld

Abstract


Introduction: The purpose of this study is to compare the cytotoxic effect of three materials which have been used for the treatment of dental hypersensitivity. Material and method: In-vitro study. Dentinal desensitizing Clinpro (3M ESPE), Seal&Protect (Dentsply) and UltraEZ (Ultradent) were used at concentrations of 0.1; 0.05; 0.01 and 0.001 g/ml against human gingival fibroblasts. Furthermore, Clinpro and Seal&Protect were applied to this cell culture as polymerized discs. Toxicity was assessed at 24 and 48 hours by cell viability assay (MTT). Statistical analysis for cell viability was performed using ANOVA two-ways and Tukey's post hoc test. Statistical significance was set at 5%. Results: Seal&Protect and Clinpro happened to be highly toxic at 24 and 48 hours, reaching 70% toxicity at concentrations over 0,01 g/ml. Seal&Protect and Clinpro polymerized discs were toxic after 24 and 48 hours. UltraEZ was increase of the cell viability between a 46% and 67% at 24 hours and between an 8% and 45% at 48 hours. Statistical analysis showed differences between these three desensitizing comparing concentration and control group (p<0,05). Discussion: UltraEZ does not have cytotoxic effect and may be considered a compatible and safe material to be utilized, whereas polymerized and non-polymerized Clinpro and Seal&Protect should be used with caution.

Keywords: “Dentin Desensitizing Agents”, “Cytotoxic”, “Human Gingival Fibroblasts”,    "Dentin Sensitivity".

Evaluación in vitro de la citotoxicidad de desensibilizantes dentinarios en fibroblastos gingivales humanos.

Introducción: El propósito de este estudio es comparar el efecto citotóxico de tres materiales que se han utilizado para el tratamiento de la hipersensibilidad dental. Material y método: Estudio in- vitro. Los desensibilizantes dentinarios Clinpro (3M ESPE), Seal&Protect (Dentsply) y UltraEZ (Ultradent) fueron utilizados a concentraciones de  0,1; 0,05; 0,01 y 0,001 g/ml sobre cultivos celulares de fibroblastos gingivales humanos. Además, Clinpro y Seal&Protect se aplicaron a este cultivo celular como discos polimerizados. La toxicidad se evaluó a 24 y 48 horas mediante ensayo de viabilidad (MTT). El análisis estadístico para la viabilidad celular se realizó mediante ANOVA de dos vías seguido de análisis Tukey. La significancia estadística se fijó al 5%. Resultados: Clinpro y Seal&Protect resultaron ser altamente tóxicos a las 24 y 48 horas, alcanzando un 70% de toxicidad a concentraciones superiores de 0,01 g/ml. Los discos polimerizados de Clinpro y Seal&Protect fueron tóxicos a 24 y 48 horas. UltraEZ produjo un aumento de la viabilidad celular entre un 46% y 67% a las 24 horas y entre un 8% y 45% a las  48 horas. El análisis estadístico mostró diferencias entre estos tres desensibilizantes al comparar la concentración y su grupo control (p<0,05). Discusión: UltraEZ no tuvo efecto citotóxico y puede ser considerado como un material compatible y seguro para ser utilizado, mientras que Clinpro y Seal&Protect en su estado polimerizado y  no polimerizado deberían ser utilizados con precaución.

Palabras clave: “Desensibilizante Dentinario”, “Citotoxicidad”, “Fibroblastos Gingivales Humanos”,  "Sensibilidad dentinaria".


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