Obtaining of the essential oil of Syzygium aromaticum, identification of eugenol and its effect on Streptococcus mutans.

Osvelia Esmeralda Rodriguez, Rosa María Sánchez, María Julia Verde, María Adriana Núñez, Rocío Castro, Abelardo Chávez


Dental caries is a disease of the oral cavity in humans. Currently seeking active principles of plants with antimicrobial effect represents a promise in Dental Therapy. This study evaluated the activity of the essential oil of Syzygium aromaticum (clove) with emphasis on its antimicrobial property. The oil was obtained by hydrodistillation, characterized by thin layer chromatography and chemical tests. The major compound was identified in the oil obtained from the flower buds and their antibacterial activity was evaluated against planktonic cells Streptcoccus mutans ATCC (700,611) performed serial dilutions; from 15 to 1000μg / mL and compared with 0.12% chlorhexidine and dimethylsulfoxide also the MIC was determined. Subsequently, the UFC was analyzed and compared with the Test CMR® Ivoclar Vivadent. The efficiency of oil extraction was 2.20%. CCD technique by the disclosed UV fraction corresponding to eugenol was identified. He had a good response to flavonoids and triterpenes. He showed greater antimicrobial activity at concentrations of 1000, 500 and 250 μg/mL. The MIC and MBC of the oil was 125 and 250 µg/mL respectively. The presence of eugenol as an active ingredient in the oil obtained was checked. Projection currently use of plant extracts has led to the evaluation of biocompatible for use in the formulations for oral hygiene products applied to the prevention or treatment of oral diseases, effective and alternative antibacterial agents.

Keywords: eugenol, Streptococcus mutans, Syzygium aromaticum, antimicrobial, minimum inhibitory concentration.

Obtención del aceite esencial de Syzygium aromaticum, identificación del eugenol y su efecto sobre Streptococus mutans.

La caries dental es una enfermedad que afecta la cavidad oral en los humanos. Actualmente la búsqueda de principios activos de plantas con efecto antimicrobiano representa una promesa en la terapia Odontológica. El presente trabajo, evaluó la actividad, del aceite esencial de Syzygium aromaticum (clavo) con énfasis en su propiedad antimicrobiana. El aceite fue obtenido por hidrodestilación, caracterizado por cromatografía en capa delgada y pruebas químicas. Se identificó el compuesto principal en el aceite obtenido de los botones florales y se evaluó su actividad antibacteriana contra células plantónicas de Streptcoccus mutans ATCC (700611) realizándose diluciones seriadas; desde 15 hasta 1000µg/mL, comparándose con clorhexidina al 0.12% y dimetilsulfóxido, además se determinó la CMI. Posteriormente, se analizó las UFC, comparándose con el Test CMR® ivoclar vivadent. La eficiencia en la obtención del aceite fue de 2.20%. Por la técnica de CCD se identificó una fracción al revelado UV, correspondiente al eugenol. Presentó respuesta positiva para flavonoides y triterpenos. Mostró mayor actividad antimicrobiana a las concentraciones de 1000, 500 y 250 µg/mL. La CMI y CMB del aceite, resultó a 125 y 250 µg/mL respectivamente. Se comprobó la presencia del eugenol como principio activo en el aceite obtenido.  Actualmente la proyección del uso de extractos de plantas ha favorecido la evaluación de agentes antibacterianos alternos, eficaces y biocompatibles para su empleo en las formulaciones de productos de higiene bucal aplicados a la prevención o tratamiento de enfermedades orales.

Palabras clave: eugenol, Streptococcus mutans, Syzygium aromaticum, antimicrobiano, concentración mínima inhibitoria.


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