Antibacterial effect of four endodontic cements against Enterococcus faecalis ATCC 29212. An in vitro study.
Abstract
Abstract: Objective: To compare the in vitro antibacterial effect of the root canal cements Endobalsam®, Top Seal®, Apexit® and Endofill® against Enterococcus faecalis ATCC 29212. Materials and method: Eighty-five applications of cements on Enterococcus faecalis, cultured in vitro on solid media in Petri dishes, were analyzed. Five groups were evaluated: four for each cement, and the fifth for the positive control (amoxicillin). The antibacterial effect was measured by the diameters of the bacterial inhibition halos at 24 hours, 48 hours, and seven days. Student´s t-test, ANOVA and the Tukey test were used for the statistical analysis. Results: No statistically significant differences were found at 24 hours (p>0.05); at 48 hours and seven days, Endofill and Apexit® had the greatest effect (p<0.05); finally, on day 7 only Endofill® showed an effect similar to the positive control (p>0.05). Conclusion: Enterococcus faecalis ATCC 29212 was susceptible to all cements. Endofill® had greater in vitro antibacterial effect than Apexit®, Top Seal® and Endobalsam®.References
1. Anumula L, Kumar S, Kumar VS, Sekhar C, Krishna M, Pathapati RM, Venkata Sarath P, Vadaganadam Y, Manne RK, Mudlapudi S. An Assessment of Antibacterial Activity of Four Endodontic Sealers on Enterococcus faecalis by a Direct Contact Test: An In Vitro Study. ISRN Dent. 2012;2012:989781.
2. AlShwaimi E, Bogari D, Ajaj R, Al-Shahrani S, Almas K, Majeed A. In Vitro Antimicrobial Effectiveness of Root Canal Sealers against Enterococcus faecalis: A Systematic Review. J Endod. 2016;42(11):1588–97.
3. Sousa BC, Gomes FA, Ferreira CM, Rocha MMNP, Barros EB, Albuquerque DS. Persistent extra-radicular bacterial biofilm in endodontically treated human teeth: scanning electron microscopy analysis after apical surgery. Microsc Res Tech. 2017;80(6):662–7.
4. Zhang C, Du J, Peng Z. Correlation between Enterococcus faecalis and Persistent Intraradicular Infection Compared with Primary Intraradicular Infection: A Systematic Review. J Endod. 2015;41(8):1207–13.
5. Sakko M, Tjäderhane L, Rautemaa-Richardson R. Microbiology of Root Canal Infections. Prim Dent J. 2016;5(2):84–9.
6. Barbosa-Ribeiro M, De-Jesus-Soares A, Zaia AA, Ferraz CC, Almeida JF, Gomes BP. Antimicrobial Susceptibility and Characterization of Virulence Genes of Enterococcus faecalis Isolates from Teeth with Failure of the Endodontic Treatment. J Endod. 2016;42(7):1022–8.
7. Vanapatla A, Vemisetty H, Punna R, Veeramachineni C, Venkata RP, Muppala JN, Dandolu R. Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics - An In-vitro Study. J Clin Diagn Res. 2016;10(4):ZC69–72.
8. Villena H. Terapia Pulpar. 1a Ed Lima, Peru: Universidad Peruana Cayetano Heredia; 2001.
9. Haghgoo R, Ahmadvand M, Nyakan M, Jafari M. Antimicrobial Efficacy of Mixtures of Nanosilver and Zinc Oxide Eugenol against Enterococcus faecalis. J Contemp Dent Pract. 2017;18(3):177–81.
10. Adil M, Singh K, Verma PK, Khan AU. Eugenol-induced suppression of biofilm-forming genes in Streptococcus mutans: An approach to inhibit biofilms. J Glob Antimicrob Resist. 2014;2(4):286–92.
2. AlShwaimi E, Bogari D, Ajaj R, Al-Shahrani S, Almas K, Majeed A. In Vitro Antimicrobial Effectiveness of Root Canal Sealers against Enterococcus faecalis: A Systematic Review. J Endod. 2016;42(11):1588–97.
3. Sousa BC, Gomes FA, Ferreira CM, Rocha MMNP, Barros EB, Albuquerque DS. Persistent extra-radicular bacterial biofilm in endodontically treated human teeth: scanning electron microscopy analysis after apical surgery. Microsc Res Tech. 2017;80(6):662–7.
4. Zhang C, Du J, Peng Z. Correlation between Enterococcus faecalis and Persistent Intraradicular Infection Compared with Primary Intraradicular Infection: A Systematic Review. J Endod. 2015;41(8):1207–13.
5. Sakko M, Tjäderhane L, Rautemaa-Richardson R. Microbiology of Root Canal Infections. Prim Dent J. 2016;5(2):84–9.
6. Barbosa-Ribeiro M, De-Jesus-Soares A, Zaia AA, Ferraz CC, Almeida JF, Gomes BP. Antimicrobial Susceptibility and Characterization of Virulence Genes of Enterococcus faecalis Isolates from Teeth with Failure of the Endodontic Treatment. J Endod. 2016;42(7):1022–8.
7. Vanapatla A, Vemisetty H, Punna R, Veeramachineni C, Venkata RP, Muppala JN, Dandolu R. Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics - An In-vitro Study. J Clin Diagn Res. 2016;10(4):ZC69–72.
8. Villena H. Terapia Pulpar. 1a Ed Lima, Peru: Universidad Peruana Cayetano Heredia; 2001.
9. Haghgoo R, Ahmadvand M, Nyakan M, Jafari M. Antimicrobial Efficacy of Mixtures of Nanosilver and Zinc Oxide Eugenol against Enterococcus faecalis. J Contemp Dent Pract. 2017;18(3):177–81.
10. Adil M, Singh K, Verma PK, Khan AU. Eugenol-induced suppression of biofilm-forming genes in Streptococcus mutans: An approach to inhibit biofilms. J Glob Antimicrob Resist. 2014;2(4):286–92.
Published
2017-12-15
How to Cite
CARRUITERO, Marcos J; VILLAVICENCIO-ROSAS, Esther.
Antibacterial effect of four endodontic cements against Enterococcus faecalis ATCC 29212. An in vitro study..
Journal of Oral Research, [S.l.], v. 6, n. 12, p. 316-318, dec. 2017.
ISSN 0719-2479.
Available at: <https://www.joralres.com/index.php/JOralRes/article/view/joralres.2017.090>. Date accessed: 24 apr. 2024.
doi: https://doi.org/10.17126/joralres.2017.090.
Issue
Section
Communications
Keywords
antibacterial effect; endodontic cement; Enterococcus faecalis.
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