Cytokines and MMPs levels in gingival crevicular fluid from patients with chronic periodontitis before and after non-surgical periodontal therapy.

  • Patrizzia Mastromatteo-Alberga Instituto de Investigaciones. Odontológicas Raúl Vincentelli, Facultad de Odontología, Universidad Central de Venezuela, Caracas, Venezuela.
  • Laura A. Escalona Cárdenas Instituto de Investigaciones. Odontológicas Raúl Vincentelli, Facultad de Odontología, Universidad Central de Venezuela, Caracas, Venezuela.
  • María Correnti Instituto de Investigaciones. Odontológicas Raúl Vincentelli, Facultad de Odontología, Universidad Central de Venezuela, Caracas, Venezuela.

Abstract

The study of host response in periodontal disease may provide a mechanism to monitor disease progression. The purpose of the present research was to determine the levels of IL-1α, IL-1β, TNF-α, IL-6, IL-6sR, IL-8, IL-10, MMP-3 and MMP-8 in gingival crevicular fluid (GCF), before and after non-surgical periodontal treatment (NSPT) in order to evaluate therapy response. Methodology: Eleven patients diagnosed with chronic periodontitis and eleven healthy subjects were selected for this study. Clinical measurements, including probing depth (PD) and clinical attachment loss (CAL) were carried out in patients diagnosed with chronic periodontitis and periodontal healthy controls. The clinical indexes evaluated were: gingival index (GI) and plaque index (PI). Samples of GCF were taken from one tooth per quadrant before and 45 days after NSPT. The levels of inflammatory mediators were measured by ELISA. Results: The values of all clinical parameters decreased significantly after treatment. The concentration levels of all cytokines and MMP-3 and MMP-8 in the GCF sample were higher in patients diagnosed with chronic periodontitis compared to the healthy group. All inflammatory mediators decreased after therapy, but did not reach control values; IL-6, Il-6sR, IL-10 and TNF-α, attained the highest reduction (70% -54%); the vales of MMP3, IL-1α, IL-1β and IL-8 were reduced between 50% - 34%; and MMP-8 showed the lowest decrease (28%). Conclusion: All clinical parameters and cytokines levels decreased after NSPT. The mediators TNF-α IL-6, IL-6sR, and IL-10 showed the largest variation between before and after NSPT and could thus be used to evaluate therapy response.

References

1. Ozgören O, Develioglu H, Güncü G, Akman A, Berker E. The adjunctive effect of tenoxicam during non-surgical periodontal treatment on clinical parameters and gingival crevicular fluid levels of MMP-8 and TNF-α in patients with chronic periodontitis - randomized, double-blind clinical trial. Adv Clin Exp Med. 2014;23(4):559–65.
2. Goutoudi P, Diza E, Arvanitidou M. Effect of periodontal therapy on crevicular fluid interleukin-1beta and interleukin-10 levels in chronic periodontitis. J Dent. 2004;32(7):511–20.
3. Shivaprasad BM, Pradeep AR. Effect of non-surgical periodontal therapy on interleukin-29 levels in gingival crevicular fluid of chronic periodontitis and aggressive periodontitis patients. Dis Markers. 2013;34(1):1–7.
4. Kaushik R, Yeltiwar RK, Pushpanshu K. Salivary interleukin-1β levels in patients with chronic periodontitis before and after periodontal phase I therapy and healthy controls: a case-control study. J Periodontol. 2011;82(9):1353–9.
5. Noh MK, Jung M, Kim SH, Lee SR, Park KH, Kim DH, Kim HH, Park YG. Assessment of IL-6, IL-8 and TNF-α levels in the gingival tissue of patients with periodontitis. Exp Ther Med. 2013;6(3):847–51.
6. Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999;4(1):1–6.
7. Kinney JS, Morelli T, Oh M, Braun TM, Ramseier CA, Sugai JV, Giannobile WV. Crevicular fluid biomarkers and periodontal disease progression. J Clin Periodontol. 2014;41(2):113–20.
8. Becerik S, Öztürk VO, Atmaca H, Atilla G, Emingil G. Gingival crevicular fluid and plasma acute-phase cytokine levels in different periodontal diseases. J Periodontol. 2012;83(10):1304–13.
9. Offenbacher S, Barros S, Mendoza L, Mauriello S, Preisser J, Moss K, de Jager M, Aspiras M. Changes in gingival crevicular fluid inflammatory mediator levels during the induction and resolution of experimental gingivitis in humans. J Clin Periodontol. 2010;37(4):324–33.
10. Tâlvan ET, Mohor C, Chisnoiu D, Cristea V, Câmpian RS. Expression of Interleukin (IL)-1β, IL-8, IL-10 and IL-13 in Chronic Adult Periodontitis Progression. Arch Med. 2017;9(34):1–8.
Published
2018-03-27
How to Cite
MASTROMATTEO-ALBERGA, Patrizzia; ESCALONA CÁRDENAS, Laura A.; CORRENTI, María. Cytokines and MMPs levels in gingival crevicular fluid from patients with chronic periodontitis before and after non-surgical periodontal therapy.. Journal of Oral Research, [S.l.], v. 7, n. 3, p. 98-101, mar. 2018. ISSN 0719-2479. Available at: <http://www.joralres.com/index.php/JOR/article/view/joralres.2018.024>. Date accessed: 27 nov. 2020. doi: https://doi.org/10.17126/joralres.2018.024.
Section
Communications

Keywords

Cytokine; Metalloproteinases; Periodontitis; gingival crevicular fluid.