Fibroblast response to initial attachment and proliferation on titanium and zirconium surfaces.

  • Araceli Meza-Rodríguez Universidad Autónoma de México.
  • Omar Martínez-Álvarez Universidad Politécnica de Guanajuato.
  • Laura Acosta-Torres Universidad Autónoma de México.
  • Javier de la Fuente-Hernández Universidad Autónoma de México.
  • René García-Contreras Universidad Autónoma de México.


Introduction: In recent decades, dental implants have become one of the best options for comprehensive dental restoration; their placement is a multidisciplinary task that requires a solid understanding of biological, periodontal, surgical and prosthetic principles. Objective: The aim of this study was to quantify in vitro the adhesion and proliferation of human gingival fibroblasts’ (HGF) response on titanium (Ti) and zirconia (Zr) surfaces. Methodology: Samples of Ti and Zr were observed under atomic force microscopy (AFM). HGFs were inoculated in each sample to determine adhesion and cell proliferation. The MTT reagent was mixed with DMEM and inoculated in each plate; formazan was dissolved with dimethyl sulfoxide and analyzed at 540 nm in a microplate spectrophotometer. The test was performed with three independent experiments. Data were analyzed with Kolmogorov-Smirnov tests (Lilliefors), Kruskal-Wallis tests and Mann-Whitney test comparisons. Results: Topography of the Zr plates showed greater roughness (Ra= 0.39μm) than Ti (Ra= 0.049μm). Quantification of HGF adhesion was significantly higher (p˂0.05) in Ti, while proliferation showed no statistically significant differences between the groups. Conclusion: It is noteworthy that, even though Ti initially showed increased cell adhesion on the surface, after 24 h Zr samples showed similar proliferation; this demonstrates that both surfaces have a comparable biological response.

Author Biography

Araceli Meza-Rodríguez, Universidad Autónoma de México.
Editor de Journal of Oral Research.Asesor en Metodología de la Investigación.


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How to Cite
MEZA-RODRÍGUEZ, Araceli et al. Fibroblast response to initial attachment and proliferation on titanium and zirconium surfaces.. Journal of Oral Research, [S.l.], v. 5, n. 5, p. 194-199, july 2016. ISSN 0719-2479. Available at: <>. Date accessed: 22 june 2021. doi:


Dental implants; titanium; zirconium; biomaterials; human gingival fibroblast.