Analysis of risk factors associated to cytomegalovirus infection in dentistry students.

Claudia De la Tejera-Hernández, Daniel Ernesto Noyola, Luis Octavio Sánchez-Vargas, Nadya Nava-Zárate, Esperanza De La Cruz-Mendoza, Araceli Gómez-Hernández, Saray Aranda-Romo

Abstract


Objective: The purpose of the study was to analyze the association between cytomegalovirus (CMV) infection in dental students with occupational risk factors and a genetic trait (NKG2C gene deletion). Study design: Case-control study. 176 students were included and divided in two groups according to CMV serological results: those with CMV infection (case group) and those without prior infection (control group). Demographic, occupational, and the presence of NKG2C gene deletion were compared between both groups. Results: The presence of CMV IgG antibodies was detected in 104 (59.1%) students (case group) while 72 (40.9%) students were CMV negative (control group). The frequency of patient contact, the use of protective barriers, and the number of reported accidents was compared between the study groups; no significant differences were noted. The appropriate use of infection-control measures was observed in the majority of students in both study groups. In the case group the frequency of NKG2C deletion was 9.7% compared to 5.6% in the control group (p=0.33). Conclusion: No association between the presence of CMV infection with occupational and genetic risk factors was found in this population. Dentists should be aware of the CMV prevalence and risks factors associated to this infection, particularly among child-bearing age dentist women.

Keywords


Cytomegalovirus; NKG2C; NK cells; occupational risk factors.

References


Griffiths P, Baraniak I, Reeves M. The pathogenesis of human cytomegalovirus. J Pathol 2015;235(2):288-97.

Muntasell A, Vilches C, Angulo A, López-Botet M. Adaptive reconfiguration of the human NK-cell compartment in response to cytomegalovirus: a different perspective of the host-pathogen interaction. Eur J Immunol 2013;43(5):1133-41.

Noyola DE, Alarcón A, Noguera Julian A, Muntasell A, Muñoz Almagro C, García J, Mur A, Fortuny C, López Botet M. Dynamics of the NK-Cell subset redistribution induced by cytomegalovirus infection in preterm infants. Hum Immunol 2015;76(2-3):118-23.

De Franca TR, De Albuquerque Tavares Carvalho A, Gomes VB, Gueiros LA, Porter SR, Leao JC. Salivary shedding of Epstein-Barr virus and citomegalovirus in people infected or not by human immunodeficiency virus 1. Clin Oral Investig 2012;16(2):659-64.

Cannon MJ, Stowell JD, Clark R, Dollard PR, Johnson D, Mask K, Stover C, Wu K, Amin M, Hendley W, Guo J, Schmid DS, Dollard SC. Repeated measures study of weekly and daily citomegalovirus shedding patterns in saliva and urine of healthy citomegalovirus-seropositive children. BMC Infect Dis 2014;14:569-79.

Swanson EC, Schleiss MR. Congenital cytomegalovirus infection: new prospects for prevention and therapy. Pediatr Clin North Am 2013;60(2):335-49.

Van Wijk PT, Meiberg AE, Bruers JJ, Groenewold MH, Van Raalten AL, Dam BA, Schneeberger PM. The risk of blood exposure incidents in dental practices in the Netherlands. Community Dent Oral Epidemiol. 2012;40(6):567-73.

Mehta A, Gupta M, Upadhyaya N. Status of occupational hazards and their prevention among dental professionals in Chandigarh, India: A comprehensive questionnaire survey. Dent Res J 2013;10(4):446-51.

Sacchetto MS, Barros SS, Araripe T de A, Silva AM, Faustino SK, da Silva JM. Hepatitis B: knowledge, vaccine situation and seroconversion of dentistry students of a public university. Hepat Mon. 2013;13(10):e13670.

Herbert AM, Bagg J, Walker DM, Davies KJ, Westmoreland D. Seroepidemiology of herpes virus infections among dental personnel. J Dent. 1995;23(6):339-42.

Garcia-Sepulveda CA, Carrillo-Acuña E, Guerra-Palomares SE, Barriga-Moreno M. Maxiprep genomic DNA extractions for molecular epidemiology studies and biorepositories. Mol Biol Rep. 2010;37(4):1883-90.

Moraru M, Cañizares M, Muntasell A, de Pablo R, López-Botet M, Vilches C. Assessment of copy-number variation in the NKG2C receptor gene in a single-tube and characterization of a reference cell panel, using standard polymerase chain reaction. Tissue Antigens. 2012;80(2):184-7.

Rangel-Ramírez VV, Garcia-Sepulveda CA, Escalante-Padrón F, Pérez-González LF, Rangel-Castilla A, Aranda-Romo S, Noyola DE. NKG2C gene deletion in the Mexican population and lack of association to respiratory viral infections. Int J Immunogenet. 2014;41(2):126-30.

Balawi F, Robertson PW, Lahara M, Rawlinson WD. Comparison of five CMV IgM immunoassays with CMV IgG avidity for diagnosis of primary CMV infection. Pathology. 2012;44(4):381-3.

Prince HE, Lapé-Nixon. Role of cytomegalovirus (CMV) IgG avidity testing in diagnosing primary CMV infection during pregnancy. Clin Vaccine Immunol 2014;21(10):1377-84

Conde-Glez C, Lazcano-Ponce E, Rojas R, DeAntonio R, Romano-Mazzotti L, Cervantes Y, Ortega-Barria E. Seroprevalences of varicella-zoster virus, herpes simplex virus and cytomegalovirus in a cross-sectional study in Mexico. Vaccine 2013;31(44):5067-74.

Cannon MJ, Schmid DS, Hyde TB. Review of cytomegalovirus seroprevalence and demographic characteristics associated with infection. Rev Med Virol 2010;20(4):202-13.

Cannon MJ, Westbrook K, Levis D, Schleiss MR, Thackeray R, Pass RF. Awareness of and behaviors related to child-to-mother transmission of cytomegalovirus. Prev Med. 2012;54(5):351-7.

Cordier AG, Guitton S, Vauloup-Fellous C, Grangeot-Keros L, Benachi A, Picone O. Awareness and knowledge of congenital cytomegalovirus infection among health care providers in France. J Clin Virol 2012;55(2):158-63.

Miyashita R, Tsuchiya N, Hikami K, Kuroki K, Fukazawa T, Bijl M, Kallenberg CG, Hashimoto H, Yabe T, Tokunaga K. Molecular genetic analyses of human NKG2C (KLRC2) gene deletion. Int Immunol 2004;16(1):163-8.


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