Bone neoformation in defects treated with fibrin platelet rich membrane versus collagen membrane: a histomorphometric study in rabbit’s femurs.

Edwin Jonathan Meza, Emil Correa, David Reynaldo Soto, Liz Katty Ríos


The aim of the present research was to compare the bone neoformation in bone defects treated with platelet rich fibrin (PRF) and collagen membrane (CM), after 3 and 5 weeks for which two bone defects were created of 4 mm width and 6 mm depth in the left femur distal diaphysis of New Zeland rabbits (n = 12). The subjects were randomly allocated in 2 groups. One of the defects was covered with a platelet rich fibrin membrane (Centrifuged resorbable Autologous blood biopolymer without biochemical modification) or collagen membrane (gold standard – Neo Mem). The second defect was left uncovered (NC). The rabbits were sacrified after 3 and 5 weeks (3 rabbits per period). The femur was completely removed and they were processed histomophometrically. The bone neoformation analysis was performed through a differential points counting. The data was statistically analyzed  (ANOVA, Tukey). The histomorphometric results showed that bone neoformation of the defects treated with PRF after 3 weeks was equivalent to the neoformation of the CM (p<0,05). After 5 weeks, the bone neoformation obtained with the PRF was higher tan the control group and lower compared with the CM bone neoformation (p<0,05).  The conclusion of the present study is that the bone neoformation of the defects treated with PRF showed lower histomorphometric results compared with the bone formation obtained with collagen membrane; and higher when compared with the control defects.


Fibrin, Bone regeneration, Collagen membrane.


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