Bone neoformation in defects treated with fibrin platelet rich membrane versus collagen membrane: a histomorphometric study in rabbit’s femurs.

Edwin Jonathan Meza, Emil Correa, David Reynaldo Soto, Liz Katty Ríos

Abstract


The aim of the present research was to compare the bone neoformation in bone defects treated with platelet rich fibrin (PRF) and collagen membrane (CM), after 3 and 5 weeks for which two bone defects were created of 4 mm width and 6 mm depth in the left femur distal diaphysis of New Zeland rabbits (n = 12). The subjects were randomly allocated in 2 groups. One of the defects was covered with a platelet rich fibrin membrane (Centrifuged resorbable Autologous blood biopolymer without biochemical modification) or collagen membrane (gold standard – Neo Mem). The second defect was left uncovered (NC). The rabbits were sacrified after 3 and 5 weeks (3 rabbits per period). The femur was completely removed and they were processed histomophometrically. The bone neoformation analysis was performed through a differential points counting. The data was statistically analyzed  (ANOVA, Tukey). The histomorphometric results showed that bone neoformation of the defects treated with PRF after 3 weeks was equivalent to the neoformation of the CM (p<0,05). After 5 weeks, the bone neoformation obtained with the PRF was higher tan the control group and lower compared with the CM bone neoformation (p<0,05).  The conclusion of the present study is that the bone neoformation of the defects treated with PRF showed lower histomorphometric results compared with the bone formation obtained with collagen membrane; and higher when compared with the control defects.

Keywords


Fibrin, Bone regeneration, Collagen membrane.

References


Hardwik R, Hayes BK, Flynn C. Devices for dentoalveolar regeneration: an up-to-date literature review. J Periodontol. 1995; 6 (6):495-505.

Hämmerle CH, Schmid J, Olah AJ, Lang NP. Osseous healing of experimentally created defects in the calvaria of rabbits using guided bone regneration. Clin Oral Implants Res. 1992; 3(3): 144 - 7.

Toffler M, Toscano N, Holtzclaw D, Corso MD, Dohan D. Introducing Choukroun’s platelet rich fibrin (PRF) to the reconstructive surgery milieu. J Implant Adv Clin Dent. 2009; 1(6): 21-30.

Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan A, Mouhyi J. Platelet -Rich Fibrin (PRF): A Second-Generation Platelet Concentrate. Part I: Technological Concepts and Evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 101 (3): 37-44.

Leigha R. Potential of platelet rich fibrin in regenerative periodontal therapy: literature review. Can J Dent Hyg. 2013; 47(1): 33-7.

Bolukbasi N, Yeniyol S, Tekkesin MS, Altunatmaz K. The Use of Platelet-Rich Fibrin in Combination With Biphasic Calcium Phosphate in the Treatment of Bone Defects: A Histologic and Histomorphometric Study. Curr Ther Res Clin Exp. 2013; 75:15-21.

Dohan DM, Bielecki T, Jimbo R, Barbe G, Del Corso M. Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a pure platelet-rich plasma (P-PRP) gel and a leukocyte and platelet-rich fibrin (L-PRF). Curr Pharm Biotechnol. 2012; 13(7):1145-52.

Dohan DM, De Peppo GM, Doglioli P, Sammartino G. Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors 2009; 27(1):63-9.

Bensaid W, Triffitt JT Blanchat C, Oudina K, Sedel L. A Biodegradable Fibrin Scaffold for Mesenchymal Stem Cell Transplantation. Biomaterials 2003; 24 (14): 2497-502.

Ríos L, Espinoza C, Alarcón M, Huamani J. Bone density of defects treated with lyophilized amniotic membrane versus collagen membrane: a tomographic and histomorfogenic study in a rabbit’s femur. J Oral Res. 2014; 3(3): 143-149.

Sunitha R, Munirathnam N. Platelet-rich fibrin: evolution of a second-generation platelet concentrate. Indian J Dent Res. 2008; 19(1):42-6.

Pripatnanont P, Nuntanaranont T, Vongvatcharanon S, Phurisat K. The primacy of platelet-rich fibrin on bone regeneration of various grafts in rabbit’s calvarial defects. J Craniomaxillofac Surg. 2013; 41(8): 191-200.

Carmagnola D, Abati S, Celestino S, Chiapasco M, Lang NP. Oral implants placed in bone defects treated with Bio-Osss, Ostims-Paste or PerioGlas: an experimental study in the rabbit tibiae. Clin Oral Implants Res. 2008; 19 (12):1246-53.

Macneill SR, Cobb CM, Rapley JW, Glaros AG. In vivo comparison of synthetic osseous graft materials a preliminary study. J Clin Periodontol. 1999; 26 (4): 239-45.

Montanari M, Callea M, Yavuz I, Maglione M. A new biological approach to guided bone and tissue regeneration. BMJ 2013; 9 (2): 1-3.

Gassling V, Purcz, Braesen JH, Will M, Gierloff M. Comparison of two different absorbable membranes for the coverage of lateral osteotomy sites in maxillary sinus augmentation: A preliminary study. J Craniomaxillofac Surg. 2013; 41(1):76-82.

Knapen M, Gheldof D, Drion P, Layrolle P, Rompen E. Effect of Leukocyte- and Platelet-Rich Fibrin (L-PRF) on Bone Regeneration: A Study in Rabbits. Clin Implant Dent Relat Res. 2013. (In press)

Yilmaz D, Dogan N, Ozkan A, Sencimen M, Ora BE. Effect of platelet rich fibrin and beta tricalcium phosphate on bone healing. A histological study in pigs. Acta Cir Bras. 2014; 29(1):59-65.

Yoon JS, Lee SH, Yoon HJ. The influence of platelet-rich fibrin on angiogenesis in guided bone regeneration using xenogenic bone substitutes: A study of rabbit cranial defects. J Craniomaxillofac Surg. 2014; 42(7): 1071-7.

Kostopoulos L, Karring T. Augmentation of the rat mandible using guided tissue regeneration. Clin Oral Implants Res. 1994; 5(2): 75-82.

Kostopoulos L, Karring T. Guided bone regeneration in mandibular defects in rats using a bioresorbable polymer. Clin Oral Implants Res. 1994; 5(2): 66-74.

Aroca S, Keglevich T, Barbieri B, Geral I. Clinical evaluation of a modified coronally advanced flap alone or in combination with a platelet-rich fibrin membrane for the treatment of adjacent multiple gingival recessions: a 6-month study. J Periodontol. 2009; 80(2):244-52.

Jankovic S, Aleksic Z, Milinkovic I, Dimitrijevic B. The coronally advanced flap in combination with platelet-rich fibrin (PRF) and enamel matrix derivative in the treatment of gingival recession: a comparative study. Eur J Esthet Dent. 2010; 5 (3):260-73.

Eren G, Atilla G. Platelet-rich fibrin in the treatment of localized gingival recessions: a split-mouth randomized clinical trial. Clin Oral Investig. 2014; 18 (8):1941-8.

Lekovic V, Milinkovic I, Aleksic Z, Jankovic S, Stankovic P. Platelet-rich fibrin and bovine porous bone mineral vs. platelet-rich fibrin in the treatment of intrabony periodontal defects. J Periodontal Res. 2012; 47(4): 409- 17.

Desarda HM, Gurav AN, Gaikwad SP, Inamdar SP. Platelet rich fibrin: A new hope for regeneration in aggressive periodontitis patients: Report of two cases. Indian J Dent Res. 2013; 24(5): 627-30.

Mazor Z, Horowitz RA, Del Corso M, Prasad HS, Rohrer MD. Sinus floor augmentation with simultaneous implant placement using Choukroun’s platelet-rich fibrinas the sole grafting material: a radiologic and histologic study at 6 months. J Periodontol. 2009; 80(12):2056-64.

Tajima N, Ohba S, Sawase T, Asahina I. Evaluation of sinus floor augmentation with simultaneous implant placement using platelet-rich fibrin as sole grafting material. Int J Oral Maxcillofac Implants 2013; 28 (1):77-83.


Full Text: PDF

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

Corresponding: Facultad de Odontología, Universidad de Concepción, Chile. Roosevelt 1550, Concepción, Chile. Phone +56-41-2204232 - Fax +56-41-2243311- Mailbox 160-C, Concepción, Chile. E-mail journal@joralres.com.