Bone density of defects treated with lyophilized amniotic membrane versus collagen membrane: a tomographic and histomorfogenic study in rabbit´s femur

  • Liz Katty Ríos Facultad de Odontología, Universidad Peruana Cayetano Heredia.
  • Carlos Vladimir Espinoza Facultad de Odontología, Universidad Peruana Cayetano Heredia.
  • Marco Alarcón Facultad de Odontología, Universidad Peruana Cayetano Heredia.
  • Jorge Omar Huamaní Facultad de Odontología, Universidad Peruana Cayetano Heredia.

Abstract

The aim of this study was to compare the bone density of bone defects treated with lyophilizated amniotic membrane (LAM) and collagen Membrane (CM), at 3 and 5 weeks. Two bone defects of 4 mm in diameter and 6 mm deep were created in left distal femoral diaphysis of New Zealand rabbits (n = 12). The animals were randomly divided into 2 groups. One of the defects was covered with lyophilized amniotic membrane (Rosa Chambergo Tissue Bank/National Institute of Child Health-IPEN, Lima, Peru) or collagen Membrane (Dentium Co, Seoul, Korea). The second was left uncovered (NC). The rabbits were killed after 3 and 5 weeks (3 rabbits/period). The results showed a high bone density and repair of the defect by new bone. The tomographic study revealed that the bone density of the defects treated with LAM at 3 weeks was equivalent to the density obtained with CM and higher density compared with NC (p <0.05). At 5 weeks, the bone density obtained with LAM was more than density CM and NC (p <0.05). The histomorphometric study showed no significant differences between LAM and CM at 3 and 5 weeks (p> 0.05). The results show that lyophilizated amniotic membrane provides bone density equal or higher to the collagen membrane.Keywords: amniotic membrane dressings, bone regeneration, Cone-Beam, Dental Implant, biological dressing.Densidad ósea de defectos tratados con membrana amniótica lifilizada versus membrana de colágeno: Un estudio tomográfico e histomorfogénico en fémur de conejo.El propósito de este estudio fue comparar la densidad ósea (DO) de defectos óseos tratados con membrana amniótica liofilizada (MAL) y membrana de colágeno (MC), a las 3 y 5 semanas. Se crearon dos defectos óseos, de 4 mm de diámetro y 6 mm de profundidad, en la diáfisis femoral distal izquierda de conejos Nueva Zelanda (n=12). Los animales fueron divididos aleatoriamente en 2 grupos. Uno de los defectos fue cubierto con  membrana amniótica liofilizada (Banco de tejidos Rosa Chambergo/INSN-IPEN, Lima, Perú) o membrana de colágeno (Dentium Co, Seoul, Korea). El segundo se dejó sin cubrir (NC). Los conejos fueron sacrificados después de 3 y 5 semanas (3 conejos/periodo). Los resultados mostraron una alta DO y reparación del defecto por hueso neoformado. El estudio tomográfico reveló que la DO de los defectos tratados con MAL a las 3 semanas fue comparable a la densidad obtenida con MC y mayor comparado con la densidad de NC (p<0,05); mientras que a las 5 semanas fue mayor a la densidad de MC y NC (p<0,05). El estudio histomorfométrico no mostró diferencias significativas entre MAL y MC a las 3 y 5 semanas (p>0,05). Los resultados muestran que la membrana amniótica liofilizada brinda densidad ósea comparable o mayor que la membrana de colágeno.Palabras claves: Apósito de membrana amniótica, regeneración ósea, Cone Beam, implante dental, apósito biológico.

Author Biography

Liz Katty Ríos, Facultad de Odontología, Universidad Peruana Cayetano Heredia.
Editor de Journal of Oral Research.Asesor en Metodología de la Investigación.

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Published
2014-06-09
How to Cite
RÍOS, Liz Katty et al. Bone density of defects treated with lyophilized amniotic membrane versus collagen membrane: a tomographic and histomorfogenic study in rabbit´s femur. Journal of Oral Research, [S.l.], v. 3, n. 3, p. 143-149, june 2014. ISSN 0719-2479. Available at: <http://www.joralres.com/index.php/JOR/article/view/joralres.2014.036>. Date accessed: 10 july 2020. doi: https://doi.org/10.17126/joralres.2014.036.
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Articles