Association of the P561T and C422F polymorphisms of the growth hormone receptor gene with facial dimensions.

  • Kazem Dalaie Department of Ortho-dontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Mohammad Behnaz Department of Ortho-dontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Samin Banihashem Private pratice, Zahedan, Iran.
  • Saeed Reza Motamedian Department of Ortho-dontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Vahid Reza Yassaee Genomic Research Center, Depart-ment of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Feyzollah Hashemi-Gorji Genomic Research Center, Depart-ment of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Arash Khojasteh Department of Oral and Maxillofacial Surgery, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

Background: Growth hormone plays a significant role in determining craniofacial morphology. Mutations of its receptor gene might be associated with mandibular prognathism (MP). Purpose: The aim of the current study was to evaluate growth hormone receptor (GHR) gene polymorphisms in relation to facial dimensions. Material and Method: The study enrolled 65 participants with class III profile in MP group and 60 orthognathic control participants. Genomic DNA was extracted from a blood sample from the patients and the P561T and C422F polymorphisms of GHR gene were screened by PCR-RFLP method followed by Sanger sequencing of randomly selected samples to validate the genotyping results. Chi square was used to compare distribution of polymorphism in MP and control groups (p<0.05). Results: Heterozygous P561T mutation was found in 10.77% and 8.33% of MP and control groups, respectively (p=0.644) while none of the subjects had the C422F mutation. Sanger sequencing confirmed the genotyping results from the PCR-RFLP method. P561T polymorphism was significantly associated with ramus and lower facial height in MP patients and with ramus height in orthognathic patients (p<0.05). Conclusion: The results indicate that the P561T polymorphism of the GHR gene is associated with the vertical dimension of the mandible in an Iranian population.

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Published
2020-02-08
How to Cite
DALAIE, Kazem et al. Association of the P561T and C422F polymorphisms of the growth hormone receptor gene with facial dimensions.. Journal of Oral Research, [S.l.], v. 8, n. 6, p. 499-504, feb. 2020. ISSN 0719-2479. Available at: <http://www.joralres.com/index.php/JOR/article/view/971>. Date accessed: 18 feb. 2020. doi: https://doi.org/10.17126/10.17126/joralres..