Comparative performance of the protocol of plasma rich in growth factors - universal 1 (PRGF-U1) for obtaining platelet rich plasma.

Augusto Aguirre, Heber Arbildo, Javier Peralta


Objective: To compare the platelet concentration obtained after application of the protocol of plasma rich in growth factors - universal 1 (PRGF-U1) and the protocol of Anitua and Andia (PRP-A) for obtaining platelet rich plasma. Material and Method: A descriptive, cross-sectional and comparative study was carried out with a simple random probabilistic sample consisting of 16 patients who attended the Periodontics service of the Unit of Second Specialization in Stomatology of the National University of Trujillo. Five blood samples were obtained from each patient, after applying a health questionnaire to rule out any disease or drug consumption, in order to obtain the baseline platelet concentration and that obtained after PRGF-U1 and PRP-A. To compare the platelet concentrations of the two protocols, Student's t-test was used considering a significance level of p <0.05. Results: The baseline platelet concentration was 371,250 ± 68,203 platelets/μL, for PRGF-U1 it was 747,875 ± 121,645 platelets/μL and for PRP-A it was 595,000 ± 129,202 platelets/μL. A statistically significant difference (p<0.001) was found between both protocols. Conclusion: The PRGF-U1 protocol yielded a higher platelet concentration compared to the Anitúa and Andía protocol.


Platelet rich plasma; Platelet-derived growth factors; Platelet count; Regenerative medicine.


Benito M, Benito M, Piletti G, González M. Plasma rico en plaquetas y su aplicabilidad en periodoncia. Una revisión. Ciencia Odontológica. 2011;8(1):44–56.

Dhurat R, Sukesh M. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author's Perspective. J Cutan Aesthet Surg. 2014;7(4):189–97.

González M, Arteaga-Vizcaíno M, Benito M, Benito M. Aplicación del plasma rico en plaquetas (PRP) y sus derivados en implantología dental y cirugía plástica. Invest Clin. 2012;53(4):408–18.

Anitua E, Prado R, Sánchez M, Orive G. Platelet-rich plasma: preparation and formulation. Operative Techniques in Orthopaedics. 2012;22(1):25–32.

Forni F, Marzagalli M, Tesei P, Grassi A. Platelet gel: applications in dental regenerative surgery. Blood Transfus. 2013;11(1):102–7.

Moreno R, Gaspar Carreño M, Jiménez Torres J, Alonso Herreros JM, Villimar A, López Sánchez P. [Methods to obtain platelet-rich plasma and osteoinductive therapeutic use]. Farm Hosp. 2015;39(3):130–6.

Marck RE, Middelkoop E, Breederveld RS. Considerations on the use of platelet-rich plasma, specifically for burn treatment. J Burn Care Res. 2014;35(3):219–27.

Anitua E, Sanchez M, Merayo-Lloves J, De la Fuente M, Muruzabal F, Orive G. Plasma rich in growth factors (PRGF-Endoret) stimulates proliferation and migration of primary keratocytes and conjunctival fibroblasts and inhibits and reverts TGF-beta1-Induced myodifferentiation. Invest Ophthalmol Vis Sci. 2011;52(9):6066–73.

Sánchez M, Anitua E, Azofra J, Andía I, Padilla S, Mujika I. Comparison of surgically repaired Achilles tendon tears using platelet-rich fibrin matrices. Am J Sports Med. 2007;35(2):245–51.

de Obarrio JJ, Araúz-Dutari JI, Chamberlain TM, Croston A. The use of autologous growth factors in periodontal surgical therapy: platelet gel biotechnology--case reports. Int J Periodontics Restorative Dent. 2000;20(5):486–97.

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